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Even though there is currently no conclusive evidence that amyloid proteins directly lead to neuronal dysfunction and cognitive decline in neurodegenerative diseases, the accumulation of amyloid proteins in the central nervous system is considered as a significant pathological characteristic of various neurodegenerative diseases, such as Alzheimers disease and Parkinsons disease [60, 61]

Consistent with the western blot data, abundant GFP-Atg8 puncta were detected following the addition of 3-IAA at a level similar to that seen following a 1-h shift to nitrogen-starvation conditions (Supplementary Fig