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Peptides were pooled in 0.5 ml Protein LoBind tubes, dried almost to completion and resuspended in 20 l solvent (3% MeCN, 0.1% TFA) with 7 l analyzed by LCMS/MS

de Jong, O

In summary, proteome and transcriptome analysis suggest a) metabolic shift and b) deranged protein homeostasis in Nf1 Myf5 mice

The connection between circulating levels of BCAAs, a marker of metabolic status, and the intake of these amino acids through diet needs to be clarified through more sophisticated experimental approaches in the future (130)