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BioMed Res Int 2016:5657491 Flocco CG, Lindblom SD, Elizabeth AH, Smits P (2004) Overexpression of enzymes involved in glutathione synthesis enhances tolerance to organic pollutants in Brassica juncea

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pH 7.5) containing protease-inhibitor mix HP (1:100 v/v) and benzonase (2 L per 10 mL lysis buffer), and then incubated on ice for 30 min before sonication using an ultrasonic homogenizer (Sonoplus HD 2070, Bandelin, Berlin, Germany)