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Ono R Review: JOURNAL OF CLINICAL MEDICINE

Next, Np and pNp were buffer exchanged to 20 mM TrisHCl pH 7.5, 150 mM sodium chloride to study intermolecular disulfide formation, which was verified by 20% SDSPAGE analysis at pH 2.5 and pH 7.5 in non-reducing conditions

This HIV-1 specificity of the suicide vector system was also supported by the observation that iRFP expression was exclusively observed in cells that were also positive for HIV-1/GFP in TNF- treated Ad-iRFP control virus transduced cells (Fig