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We were therefore unable to determine the relative contributions of glutamine and glutamate to the lower levels of Glx/Cr observed on NAC administration, although this would be possible in future studies using 1 H-MRS optimised for detection of glutamate and glutamine

Differential methylation of splenic tissue and WBCs has been demonstrated in PI calves at day 245 of gestation and 4 months of age, respectively [24, 25]