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Yeh JT, Yu YC, Hwang TC (2019) Structural mechanisms for defective CFTR gating caused by the Q1412X mutation, a severe class VI pathogenic mutation in cystic fibrosis

the supernatant was collected and frozen at -20C, for future chemical analyses

The Inter-Organization Programme for the Sound Management of Chemicals (IOMC) was established in 1995 by UNEP, ILO, the Food and Agriculture Organization of the United Nations, WHO, the United Nations Industrial Development Organization, the United Nations Institute for Training and Research, and the Organisation for Economic Co-operation and Development (Participating Organizations), following recommendations made by the 1992 UN Conference on Environment and Development to strengthen cooperation and increase coordination in the field of chemical safety

Centrifuge anticoagulant-treated blood at 1,000 g for 10 min at 4C