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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

The CellTiter-Glo 2.0 Assay uses a single, ready-to-use liquid reagent that eliminates the substrate reconstitution step required with the original two-component kit

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FGF21 prevents neuronal cell ferroptosis after spinal cord injury by activating the FGFR1/beta-Klotho pathway