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Due to the peptide compositions, AMPs are unfortunately limited in their potential to be used in vivo by a number of variables, including minimal tissue penetration, rapid removal from circulation, decreased bioavailability, limited stability, and low selectivity against bacterial cells relative to mammalian cells

For instance, circulation-related pain might need medication or surgery, while muscle or nerve pain may respond to physical therapy

This ensures maximum bioavailability and effectiveness

The review article details analytical challenges such as heterophilic antibodies, pituitary HCG in peri and postmenopausal women, and variant forms that may cause false positive or negative results, guiding assay design and interpretation