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Fluorescence signals were visualized using a fluorescence microscope (Nikon Ts2R) and quantified by flow cytometry (NovoCyte 3000, Agilent, USA)

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Since cellular senescence is considered a natural defense mechanism against tumor development, and senescence inductions triggered by currently applied therapies can significantly impact their effectiveness, it is assumed that purposeful stimulation of senescence in cancer cells may be a promising direction in the development of anticancer therapy [8, 15, 55]

No evidence of hormonal, hepatic, or systemic adverse effects has been observed in available data