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Depending on the desired culture volume and the planned duration of cultivation, the culture vessels used were either shake flasks (100, 250, or 500 mL) sealed with a metal cap and lab tape to reduce evaporation or 240-mL amber screw cap Boston bottles (Sigma-Aldrich, SKU #23235) with a screw cap with a central hole for a PTFE septum (Sigma-Aldrich, SKU #27022), which were tightly sealed to prevent evaporation completely

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YAP1 induces bladder cancer progression and promotes immune evasion through IL-6/STAT3 pathway and CXCL deregulation

The vant Hoff Factor in Detail The dissociation factor (i), also called the vant Hoff factor after the Dutch chemist Jacobus Henricus vant Hoff who derived the osmotic pressure equation in 1887, is more subtle than it first appears