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Acute myeloid leukemia (AML) cell lines (CMK, GFD-8, HEL, K562, Kasumi-1, KBM3, KBM5, KCL22, KG1a, KU812, KY0-1, LAMA-84, M07e, MOLM-13, MOLM-14, Monomac-1, NOMO-1, OCI-AML2, OCI-AML3, OCI-AML5, SET2, SKM-1, SKNO-1, TF-1, THP-1) were grown in RPMI supplemented with 10% FBS (Sigma), 1% Glutamax (ThermoFisher), and 100 U/mL penicillin/streptomycin (ThermoFisher)

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The Na + -H + exchanger is key for regulating normal pH of ECs, and the Na + -Ca 2+ exchanger maintains a low level of intracellular Ca 2+ in ECs (Sweeney et al., 2019b)

However, a deeper mechanistic understanding of both processes is required to validate POS as a novel example of physiological conditioning hormesis