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(4 m) were prepared using a microtome, and the TUNEL assay was performed on brain tissue following the manufacturers instructions provided by Bio vision (Milpitas, USA)

Using antibodies, Tsai and Lin (2007) observed V-ATPases in apical membranes of the gills of Eriocheir sinensis and four other crab species from the Ocypodidae and Grapsidae families, whereas the V-ATPases were located in the cytoplasm in some other species (for a possibly function of cytoplasmic V-ATPase see also below in the nitrogen excretion section)

maculopapular, erythematous, or eczematous eruptions

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