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Chronic opioid use suppresses cortisol release, altering the brains ability to respond appropriately to stress

Through DDT, ssDNA breaks are bypassed via either an error-prone mechanism triggered by mono-ubiquitination of proliferating cell nuclear antigen (PCNA) and trans-lesion synthesis, or an error-free lesion bypass mechanism involving template switching to the undamaged strand and requiring polyubiquitination of PCNA (Fig