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Decreased serum calcium stimulates parathyroid hormone secretion, leading to increased osteoclast activity, which in turn exacerbates bone destruction (59)

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In summary, these findings support both the stimulation and inhibition of adipocyte lipolysis for therapeutic benefit, which will need to be specifically tailored to the disease state in question (Table 1)

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Transient human MC4R transfected HEK293T cells were pelleted, washed and resuspended in stimulation buffer (LANCE Ultra cAMP assay) supplemented with 0.1% BSA according to the manufacturers instructions

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