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Moreover, the discovery of IRAP inhibitors by virtual and substance library screening and direct design applying knowledge of the structure of IRAP and of related enzymes is briefly presented

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The levels of samples AGEs (Sigma-Aldrich, MO, USA), TNF- (Sigma-Aldrich, MO, USA), IL-6 (Sigma-Aldrich, MO, USA) and collagen-I (Sigma-Aldrich, MO, USA) were quantified using the enzyme linked immunosorbent assay (ELISA) detection kit according to the manufacturers instructions