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Description

In this dot blot approach, a gDNA to be detected was spotted directly onto a membrane as a dot, and then membranes blocked and probed with appropriated 5 mC or 5 hmC antibodies

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Regular IGF-1 has 70 amino acids, while Long R3 IGF-1 has 83 amino acids due to the addition of 13 amino acids on its N-terminal end

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