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Sulfate uptake assay The DNA fragment encoding the six Histidine (6XHIS) tag with a polylinker (5-AATTCTCTAGAGAGCTCGTCGACTTCACCACCACCACCACCACTAAAAGCTTCTC GA-3) was ligated to the EcoRI and SalI digested pYX222x plasmid (Shibagaki et al., 2002)

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An imbalance between the pro-death and pro-survival proteins (also known as pro-apoptotic and anti-apoptotic proteins, respectively) can trigger downstream proteins in this pathway, leading to DNA fragmentation and cell death