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10.1016/B978-0-12-407697-6.00002-7 135

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In 1980, the US Congress held hearings on claims that the FDA was slow in approving DMSO for other medical uses

Key readout endpoints include serum ALT and AST enzyme release (hepatocyte membrane integrity), hepatic malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) as lipid peroxidation markers, H&E-stained hepatic necrosis area scoring (zones IIII centrilobular pattern), TUNEL-positive hepatocyte apoptosis count, and MPO activity as a proxy for neutrophil infiltration