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250 l of PBS containing human HGF (1.25 ng) were incubated with 3 H-Hinge, the central dimerization domain of HGF, in the presence of varying concentrations of 6-AH analogs between 10 13 M to 10 7 M (half-log dilutions) for 40 min at 37 C

The overall molecular properties of orforglipron, molecular weight approximately 529 Da, moderate lipophilicity (cLogP approximately 3.5), and limited hydrogen bond donors and acceptors, place it within the "drug-like" chemical space defined by Lipinski's Rule of Five, although at the upper boundary