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Following DNA isolation, unmethylated cytosine nucleotides were converted into uracil with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, California)

There are also earlier time-point observations in the literature

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Additional studies found that elimination of hepatic IGF-1 gene expression results in a compensatory threefold increase in GH secretionrecall that IGF-1 is secreted by GH stimulation in hepatocytes