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Sixteen hours after transfection, biotinylation was triggered by adding biotin to a final concentration of 50 M (1 mM biotin stock in PBS and stored for up to 6 months)

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Patient cells are transduced with a viral vector in a laboratory setting (typically retrovirus or lentivirus) with the result being a set of gene-modified cells carrying copies of the functional gene that can be readministered to the patient