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Description

qPCR primers were designed using a primer design tool, and their sequences were as follows: Pkm1 , forward 5-CACCGTCTGCTGTTTGAAGA-3, reverse 5-TCAAAGCTGCTGCTAAACACTT-3

After preparing pH of the medium (~6.8) to 6.9 by adding ammonia solution (28%) (Nacalai Tesque), the culture was started by adding the above-mentioned inoculum into the medium