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As per studies, it has been reported that basal GH levels may increase by 7.5-fold, which appears to have contributed to an overall increase GH secretion by 46% (1) Though acting on different receptors, research suggests that synergistically, these peptides may promote pulsatile release of growth hormones that is subject to negative feedback, and may prevent supratherapeutic levels of GH and their sequelae (5) It has been hypothesized that GHRH-mimetics such as CJC-1295 and the secretagogue GHRP-2 may potentially exhibit synergistic effects in regard to their apparent stimulation of growth hormone secretion

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EdU staining A 5-ethynyl-2-deoxyuridine (EdU) incorporation assay was conducted using a kFluor647 Click-iT EdU Imaging Test Kit (KeyGen BioTec, Nanjing, China), according to the manufacturers protocol

doi:10.18632/aging.102281