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The RT-PCR products were then visualized by electrophoresis on a 2% (v/v) agarose gel under UV light

One of the primary mechanisms postulated to underlie the neurodegenerative effects of CCL11 in long COVID involves the induction of hippocampal microgliosis, leading to diminished neurogenesis and the deterioration of myelinated subcortical axons 27

Immunoblotting analysis Equal amounts of protein were separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred onto polyvinylidene difluoride membranes