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The enzyme concentration was subsequently determined by UVVis spectroscopy using an extinction coefficient of 51,340 Mcm, which was calculated using Expasy ProtParam ( The oxygen consumption assay was performed using an oxygraph (Hansatech Instruments), which monitors oxygen uptake or evolution in real time using an S1 Clark-type electrode

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Methylcobalamin 2500mcg is often prescribed to treat vitamin B12 deficiency , which can lead to various health issues such as fatigue, weakness, memory problems, and nerve-related symptoms like numbness or tingling in the hands and feet

Future work should move beyond qualitative claims of biodegradability toward quantitative degradation kinetics and body-burden profiles in human-relevant models, so that nanocarriers can be compared on the basis of standardized end-of-life fingerprints across organs and time scales