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Immunofluorescence and Microscopy For immunofluorescence analysis, 8x10 4 cells (12-well plates) were grown on glass coverslips coated with type I collagen (0.5 mg/ml) or fibronectin (0.1 mg/ml), fixed with 3.7% formaldehyde in PBS for 15 min, and permeabilized with 0.2% (v/v) Triton X-100 in PBS for 10 min

Total RNA was extracted from rice tissues following the protocol established by Chen et al

Access this chapter Subscribe and save Buy Now Tax calculation will be finalised at checkout Purchases are for personal use only Similar content being viewed by others References Anestal K, Prast-Nielsen S, Cenas N, Arner ES (2008) Cell death by SecTRAPs: thioredoxin reductase as a prooxidant killer of cells

CRISPR-cas9-mediated gene editing of MYB28 genes impair glucoraphanin accumulation of brassica oleracea in the field