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Folate may be more important for rapidly dividing tissue, like the colonic mucosa

Sun, C., Wang, A., Zhou, Y., Chen, P., Wang, X., Huang, J., Gao, J., Wang, X., Shu, L., Lu, J., Dai, W., Bu, Z., Ji, J., & He, J

PMID: 40 LiuYLuSWuLYangLYangLWangJ

In addition, with membrane capacitance measurements, one can monitor activity-dependent SV dynamics, consisting of exocytic increases of membrane capacitance and subsequent decay due to compensatory endocytosis of exocytosed SV membranes (Sun and Wu, In addition to electrophysiologically amenable preparations of the calyx of Held synapses in acute brainstem slices, these giant presynaptic terminals were successfully reconstituted by culturing two types of dissociated cells derived from cochlear nuclei and from medial nuclei of the trapezoid body in the same dish (Dimitrov et al., Manipulation of Vesicular Glutamate Content by Dialyzing Presynaptic Terminals with Solutions Containing Various Glutamate Concentrations Direct access to the giant presynaptic terminal of the calyx of Held using glass pipets allows us to clamp the presynaptic cytoplasm with a solution of the desired composition