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and, (c) using the set of mutagenic oligonucleotides to generate a set of major histocompatibility complex (MHC) molecule- encoding variant nucleic acids encoding a range of amino acid variations at each amino acid codon that was mutagenized, thereby producing a library of nucleic acids encoding a plurality of variant major histocompatibility complex (MHC) molecules

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reperfusion) or across renal cell subsets (e.g., tubular epithelial cells, glomerular cells, and immune cells) has not been systematically dissected