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Then, 1.5 10 6 cells were seeded into a six-well plate in 2.5 ml of SILAC medium (typically two wells per condition)

(PubMed) Shi Z, Zhen S, Wittert GA, Yuan B, Zuo H, Taylor AW

Upon activation, NLRP3 oligomerizes and recruits ASC via PYD-PYD binding, forming a scaffold that transports procaspase-1 molecules into proximity through the CARD-CARD interaction