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The upregulation of HO1 is highly related to the increment of Fe 2+ in LIP, as HO1 is able to promote the release of Fe 2+ in heme (53)

Effects of trace mineral supplementation in lactating dairy cattle

After 180 days, the specimens were again stored in a desiccator with silica gel and weighed every 24 h until the daily weight difference fell below 0.1 milligrams, indicating that equilibrium of the specimen mass was reached, which was noted as the final mass (m 3 )

However, due to the complexity and ethical constraints of clinical trials, it is difficult for the latter to conduct large-scale and long-term follow-up, and in contrast, most clinical RCTs aim at the validation of symptomatic drugs such as calcium, vitamin D, and BPs, while the microecological modifiers that target the gutbone axis are seldom screened