blood oxidized glutathione Reduced (GSH) (a) and glutathione (GSSG) (b) Time-course of glutathione oxidation and

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Precise control of operational parameterssuch as power, temperature, and extraction durationis essential, as excessive energy input or prolonged treatment can degrade sensitive polyphenolic structures, potentially lowering yield or altering compound profiles ( 3.1.4 Supercritical fluid extraction method Supercritical fluid extraction is an alternative two-step technique

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It emphasises gene interpretation and differential gene expression analysis (DGE), which is always its standard research tool, suitable for gene expression difference studies and pathway analysis of entire tissues or cell populations 12,13