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95 Cysteine is a semi essential amino acid as it can be produced from methionine through the transsulfuration pathway converting methionine to homocysteine, cystathionine, and in a final step to cysteine

Electron microscopy sample preparation and data acquisition A 3Ll volume of 5LmgLml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)

Acknowledgments Infrastructural part of this project has been supported by CZ.1.05/2.1.00/01.0030 (Biomedreg)