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Description

Approximately 50 mg of cerebral cortex tissue was homogenized in RIPA lysis buffer supplemented with a protease inhibitor cocktail (Santa Cruz Biotechnology, sc-24948, USA), followed by centrifugation at 16,000 g for 20 min at + 4 C to obtain the supernatants

M.LiH.et al (2015)

The amphipathic structure of melatonin allows easy access to different tissue cells and their organelles, interacting with intracellular molecules with no interaction with receptors, exerting in this way an antioxidant action