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Disulfide bond formation in the cytoplasm

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Mechanistically, mitochondrial dysfunction, as discussed above, is an increasingly recognized contributor, particularly in diabetic nephropathy, in which mitochondrial ROS production exceeds the local antioxidant capacity [13,34]

Additional DNase digestion (Thermo Scientific) and purification was applied (RNA Clean and Concentrator-25 Kit, Zymo Research), and cDNA was synthesized from 1 g of total RNA using RevertAid reverse transcriptase (Thermo Scientific) and random hexamers