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doi: 10.2353/ajpath.2009.090417

Seperti yang diketahui, kolagen berperan penting dalam membuat kulit tetap kenyal, halus, dan terhidrasi Jika produksi kolagen menurun, maka elastisitas kulit akan berkurang yang berujung pada munculnya keriput dan kulit tidak kencang lagi

The plate was then incubated at 37 C for 45 minutes in a shaker water bath then cooled and the absorbance of the pink colored chromophore was measured at 540 nm using ELISA reader, against a blank treated in a similar manner to the test but using 100l distilled water instead of the sample