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Huachansu Injection is derived from the dried skin of the Bufo gargarizans and has traditionally been used for its detoxifying, anti-inflammatory, and analgesic properties

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Despite its therapeutic potential, the clinical use of QCN is hindered by poor water solubility, chemical instability, and limited dermal penetration, all of which result in low bioavailability

Animal Toxicology Studies Animal toxicology studies tested doses from 0.0027 mg/lb to 9.1 mg/lb via intraperitoneal, intramuscular, intravenous, and oral routes for up to 6 weeks, finding: No acute toxic or lethal dose No gross or histologic toxicity in major organs No hepatotoxicity or nephrotoxicity Negative Ames test (no mutagenicity) Negative chromosomal aberration tests (no genotoxicity) No teratogenicity in rat pregnancy studies No local injection site irritation The LD1 (minimum lethal dose) could not be achieved despite aggressive testing