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To clarify which types of polyubiquitin chain were added to HMOX2 by MITOL, a ubiquitin chain restriction assay using UbiCRest, which utilizes ubiquitin chain-specific deubiquitinating enzymes, was performed 30

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falciparum parasites (here shown for the 3D7 strain) expressing hGrx1-roGFP2 were treated (after 60 s baseline monitoring) with 1 mM diamide and monitored for 4 min