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An aliquot (100 L in volume) was withdrawn, then diluted with methanol up to 1 mL, thoroughly mixed, followed by centrifugation for 10 min at 15,000 rpm

Experimental procedures for isolated tubules: Tubules were suspended at 1.5-2.0 mg tubule protein/ml in a 95% air/5% CO 2 -gassed medium containing (in mM) 110 NaCl, 2.6 KCl, 25 NaHCO 3 , 2.4 KH 2 PO 4 , 1.25 CaCl 2 , 1.2 MgCl 2 , 1.2 MgSO 4 , 5 glucose, 4 sodium lactate, 0.3 alanine, 5 sodium butyrate, 2 glycine, and 1.0 mg/ml bovine gelatin (75 bloom) (Solution B)

Rule Out Other Causes If gas persists, consider whether other factors (like diet, lactose intolerance, or gut health issues) may be contributing

If you have any medical conditions, please consult a medical professional before buying