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Thermo Fisher Scientific) with 10% FBS and without antibiotics

The inconsistency in diagnostic standards and case definitions for ME/CFS poses a major obstacle to its clinical diagnosis

Specifically, in the presence of TGF1 plus L-carnitine, there was a slight reduction in SMA-positive cytoplasmic staining, whereas TGF1 plus CPZ resulted in a more prominent reduction in cytoplasmic SMA immunostaining