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Mouse PrP cDNA constructs carrying a deletion of the OR region (OR), or in which the histidines in the OR (Ala1) or in the OR and non-OR region (Ala2) was substituted with alanines, or in which the glycine in position 53 was substituted with a serine (G53S), were synthesized by GeneArt (Thermo Fisher) and inserted between the HindIII and BamHI restriction sites of pCDNA3.1(+)

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Anaphylactic shock and death have been reported after parenteral Vitamin B12 administration