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The resulting plasmid pQE-BBK32 served as template and oligonucleotides BBK32-205 BamHI and pQE-RP (Table S5) for a subsequent PCR amplification to engineer a His-tagged BBK32 205 fragment containing the C-terminal amino acids 205 to 356

Coupling reactions The biochemical conjugation between polymeric NPs and biomolecules can be performed using coupling agents (Figure 4F)