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This is a preview of subscription content, access via your institution Access options Access Nature and 54 other Nature Portfolio journals Get Nature+, our best-value online-access subscription 17.99 / 30 days cancel any time Subscribe to this journal Receive 1 digital issues and online access to articles 99.00 per year only 99.00 per issue Buy this article Purchase on SpringerLink Instant access to the full article PDF

Cerny, M

The vector, pET 15b (Figure 1A-1), was constructed by inserting a flexible spacer sequence, a hexa-histadine tag and thrombin cleavable sequence (Figure 1B, top)