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Results A TLR3-based directed evolution approach identifies an NS3 helicase point mutant attenuating HCV replication and ablating TLR3 activation Since TLR3 is the main sensor for dsRNA in HCV infected cells, leading to ISG induction and reduction of HCV replication 30,31,32,33 , we performed a directed evolution approach aiming at identifying viral determinants of TLR3 activation (Fig

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