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Once converted, vitamin E acts as a skin's main fat-soluble antioxidant that fights free radicals to protect skin from damage

Carbohydrate metabolism pathways were inhibited by Ni 2+

Methods Biological resources All mammalian cell lines were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% foetal calf serum (Seromed, inactivated for 30 min at 56 C), 100 U/mL penicillin and 100 g/mL Streptomycin, 2 mM L-glutamine (Gibco) and 1% 100x non-essential amino acids (Gibco) (DMEM complete) at 37 C and 5% CO 2

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