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A second nested-PCR enrichment step was performed for 11 cycles using the same cycling parameters as before, using the entire bead-purified reaction product from the first step as a template with primers YL009 (CAAGCAGAAGACGGCATACGAG) and JBD-SS-273:280 (AATGATACGGCGACCACCGAGATCTACAC TATAGCCT ACACTCTTTCCCTACACGACGCTCTTCCGATCT NNNNNNN GCAGGGATGTCCACGAGGTCTCT) where N 8 barcodes correspond to reverse-complemented Illumina TruSeq HT D501-508 indexes

Briggs, S

Dutch researchers used MRI scans to study the brains of 10 men in their mid-20s who had taken an average of 281 ecstasy tablets over the previous six and a half years, and seven peers who had taken other drugs