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It is important to note that these metabolic responses to SAAR are not confined to rodents

Interestingly, MeCbl blocks GSDME-mediated pyroptosis, but barely has any effect on apoptosis when Gsdme genetic deficiency cells were challenged with apoptotic stimulation

1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

The process is straightforward but requires attention to detail