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Due to this dual mechanism, even a small amount of GSH is sufficient to maintain thiol groups in their reduced state [51, 52]

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Tissue sections were blocked with 3% goat serum for 60 minutes at room temperature, stained with an antibody cocktail solution (containing 750 g/mL of each antibody) at 4C for 15 hours, washed with 0.2 % Triton X-100 in PBS for 8 minutes, and then stained with nuclear Intercalator-Ir (Fluidigm) at 300 L/section