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The acute decrease in UMP/UDP/UTP levels that resolves despite continued TS inhibition supports the hypothesis that an increase in nucleotide salvage might be linked to our observed mitochondrial phenotypes

Cancer Growth Metastasis 2017, 10:1179064417696070

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Each freeze-thaw cycle stresses the peptide in solution and can cause aggregation, the process by which peptide molecules clump together and lose biological activity