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The body struggles to transport sufficient amounts of oxygen to its various tissues, which can cause debilitating weakness

We are given [base] = [Py] = 0.119 M and [acid] = [HPy + ] = 0.234 M

Purification and Detection High performance liquid chromatography (HPLC) purification: The synthesized polypeptides need to be purified by HPLC to remove impurities and unreacted raw materials, ensuring that the purity meets the pharmaceutic-grade standard

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