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Functionally, in the SLC30 family, ZnT1 functions primarily as a zinc exporter on the cell membrane, transporting cytoplasmic zinc ions across the membrane to the extracellular space, while other ZnT proteins are situated on the membranes of intracellular organelles

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Insoluble material was removed by centrifugation at 65,000 g for 40 min and the solubilized complex was incubated overnight at 4 C with pre-equilibrated Nickel resin (Ni Smart Beads 6FF, SMART Lifesciences) containing 10 mM imidazole